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A cell culture plate is a cylindrical vessel used for culturing cells

A New Type of Cell Culture Plate
A cell culture plate is a cylindrical vessel used for culturing cells. The shape, size and treatment of a cell culture plate affect the seeding, adherence, and growth of the cells. These effects are important for biomedical research and for in vitro assessment of biological materials.
In the context of tissue engineering, several types of cell culture plates have been tested and evaluated. However, the geometrical properties of these vessels have been limited. Therefore, it is necessary to find a method that enables the researcher to control the geometry of the cell culture plate. One possible solution is a multi-well plate that can accommodate a series of single wells. This allows researchers to create a compact three-dimensional structure that can be used for a variety of applications.
Some of these plates have been treated with various chemicals to enhance cell adhesion. For example, a polyethyleneimine (PEI) coating helps inhibit non-specific binding. On the other hand, an aluminum alloy has been found to be a viable surface treatment that resists mechanical actions. Another option is the membrane-bottom type. It can be used with a multi-well feeding tray. Both of these are viable options for cell suspension cultures.
Cells preferentially attach to the bottom surface of a culture plate. This may be due to gravity or the presence of an organic coating. Alternatively, a poly-lysine coating may be used. But, this method is also problematic because the coating interferes with negatively-charged ligands. Lastly, a chimney well plate helps reduce the potential for contamination.
The process of preparing a cell culture plate is not an easy one. It requires a high degree of expertise and a large investment. To get an idea of the complexity of this task, we studied a new plate designed to prepare 3D cell spheroids. While this method is still in its infancy, it has the promise of producing cell spheroids with viability and durability. Moreover, it is easier to prepare than a conventional tissue culture plate. Consequently, this method could prove to be the ideal choice for cell culture experiments.
The newly-developed plate was manufactured using computer-aided design and manufacturing software. This process enables the researcher to quickly and easily design and build a plate for mass production. Using this method, the researcher can design the efficient and affordable cell culture plate. After the design was finalized, the plate was fabricated by pouring the PDMS into a machined base frame.
The cells in the newly-fabricated cell culture plate were able to form spheroids within 24 hours of incubation. They adhered to the scaffold fibers and closed pores by day 14. When the cells were analyzed, it was found that they had similar morphology and viability to those in a commercially-available U-plate. Additionally, this plate has the ability to produce cell spheroids in a high volume. Hence, it holds the promise of becoming a practical tool in a number of applications.
Other surface treatments can improve cell attachment. However, they can also to non-specific binding. Therefore, it is critical to conduct biocompatibility studies. These tests are particularly important for in vitro assessment of scaffolds.

Cell Culture Plate
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